Recent Scientific Articles Regarding Cryopreservation

Where does research currently stand?

Where do we stand on the development of cryopreservation

Winter is coming: the future of cryopreservation

  • Organ and tissue preservation could facilitate the transplantation process and solve the problems connected to the shortage of organ donors — by allowing the creation of an organ bank. The need for such a solution is high. In fact, in 2010 only 10% of the worldwide need for organ transplantation was met.
  • Fertility preservation (eggs, sperm, or reproductive tissue) is a field that has grown exponentially, with great results. Since the first birth following in vitro fertilization (IVF) in 1978, at least 8 million babies have been produced with the help of medically assisted reproduction.
  • Drug discovery, development and testing could also benefit from the availability of large quantities of cryopreserved tissues and cells. Pharmaco-toxicological research could utilize tissue slices of human organ material. This would lead to more effective results and a decrease of laboratory animal use.
  • Stem cells represent highly promising resources for application in cell therapy and regenerative medicine. Their commercial and clinical applications, presently, depend on cryopreservation — the only available long-term storage option.
  • Finally, human cryopreservation could completely revolutionize the field of emergency medicine. The use of very low temperatures to save lives is still in its infancy. Moderate states of hypothermia have recently become a common practice to gain crucial time in emergency situations. Depth hypothermia and vitrification are less common, yet promising fields.
More than 5000 cardiac transplants occur each year around the world, although it is estimated that up to 50.000 people are candidates for transplantation

New approaches to cryopreservation of cells, tissues, and organs

  • Supercooling. Many species in nature can sustain subfreezing body temperatures for weeks or longer, supercooling/hibernating to avoid ice formation. Supercooling could be artificially induced in human organs through the application of low-molecular-weight cryoprotectants and synthetic ice blockers.
  • Controlled, Partial-Ice Freezing. There are at least 45 animals that can survive long periods of time at high-subzero temperatures in a state of biostasis. Researchers are studying low-molecular-weight CPAs that could help reproducing this ability in humans.
  • Nanowarming. In order to accomplish both rapid and uniform warming after cryopreservation, we could use heat transfer methods capable of warming tissue from within rather than through surface conduction alone. Nanowarming technology could do so by biocompatible magnetic nanoparticles perfused with the vitrification solutions. Through radiofrequency fields we could excite these nanoparticles, causing a rapid and uniform warming.
Successful nanowarming technology would likely solve revival — Credit image: ResearchGate

Vitrification and Nanowarming of Kidneys

Cryopreservation of a human brain and its experimental correlate in rats

Conclusion

References

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Tomorrow Biostasis GmbH

We are a Berlin based longevity company committed to advancing Biostasis technology and promoting it in a simple and transparent way. www.tomorrowbiostasis.com